ssr_primer
Primer Details for TRINITY_DN65543_c2_g2_i5 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGCGTTTCGTTTTGAGCT | CGTGGAAACAGAGACAGGGG | Start: 66 |
| Tm | 59.971 | 60.321 | End: 339 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | AGTGGCGTTTCGTTTTGAGC | CGTGGAAACAGAGACAGGGG | Start: 65 |
| Tm | 59.971 | 60.321 | End: 339 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | GTGGCGTTTCGTTTTGAGCT | ACGTGGAAACAGAGACAGGG | Start: 66 |
| Tm | 59.971 | 59.607 | End: 340 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
|---|
| Sequence | AGTGGCGTTTCGTTTTGAGC | ACGTGGAAACAGAGACAGGG | Start: 65 |
| Tm | 59.971 | 59.607 | End: 340 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | CGATTCGGAAAGCCTCTCGT | CTAGTCATAACCGGCGTGGG | Start: 414 |
| Tm | 60.179 | 60.249 | End: 611 |
| Size | 20 | 20 | 198 |
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