ssr_primer
Primer Details for TRINITY_DN65521_c2_g8_i1 |
|---|
| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGGAAATCGGCCGGAAAT | TTTTTGAGCATTTCCGGCCG | Start: 216 |
| Tm | 60.034 | 60.039 | End: 430 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GGACCTCCCTCGTTTGGATC | ATTTCCGGCCGATTTCCACT | Start: 135 |
| Tm | 59.822 | 60.034 | End: 235 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | AGTGGAAATCGGCCGGAAAT | ATTCCTTCCAGATCCGTGGC | Start: 216 |
| Tm | 60.034 | 59.819 | End: 403 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | AAGTGGAAATCGGCCGGAAA | TTTTTGAGCATTTCCGGCCG | Start: 215 |
| Tm | 60.251 | 60.039 | End: 430 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | AAAGTGGAAATCGGCCGGAA | TTTTTGAGCATTTCCGGCCG | Start: 214 |
| Tm | 60.251 | 60.039 | End: 430 |
| Size | 20 | 20 | 217 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGGAAATCGGCCGGAAAT | TTTTTGAGCATTTCCGGCCG | Start: 216 |
| Tm | 60.034 | 60.039 | End: 430 |
| Size | 20 | 20 | 215 |
| Primer 2 details |
|---|
| Sequence | GGACCTCCCTCGTTTGGATC | ATTTCCGGCCGATTTCCACT | Start: 135 |
| Tm | 59.822 | 60.034 | End: 235 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | AGTGGAAATCGGCCGGAAAT | ATTCCTTCCAGATCCGTGGC | Start: 216 |
| Tm | 60.034 | 59.819 | End: 403 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | AAGTGGAAATCGGCCGGAAA | TTTTTGAGCATTTCCGGCCG | Start: 215 |
| Tm | 60.251 | 60.039 | End: 430 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | AAAGTGGAAATCGGCCGGAA | TTTTTGAGCATTTCCGGCCG | Start: 214 |
| Tm | 60.251 | 60.039 | End: 430 |
| Size | 20 | 20 | 217 |
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