ssr_primer
Primer Details for TRINITY_DN65510_c4_g1_i5 |
|---|
| (T)22: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGAAAGACCCCTAACTCC | AATGACCAGACTGCAGGAGC | Start: 202 |
| Tm | 60.108 | 60.036 | End: 475 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGAGCCGAAAGACCCCTAAC | AATGACCAGACTGCAGGAGC | Start: 199 |
| Tm | 60.108 | 60.036 | End: 475 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | CGCTTGCGCACCAAATGATA | GTTAGGGGTCTTTCGGCTCC | Start: 82 |
| Tm | 59.901 | 60.108 | End: 218 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CGCTTGCGCACCAAATGATA | GGAGTTAGGGGTCTTTCGGC | Start: 82 |
| Tm | 59.901 | 60.108 | End: 221 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | GCTCCTGCAGTCTGGTCATT | TAGAGCAATCGAAGCCACGG | Start: 456 |
| Tm | 60.036 | 60.179 | End: 567 |
| Size | 20 | 20 | 112 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGAAAGACCCCTAACTCC | AATGACCAGACTGCAGGAGC | Start: 202 |
| Tm | 60.108 | 60.036 | End: 475 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | GGAGCCGAAAGACCCCTAAC | AATGACCAGACTGCAGGAGC | Start: 199 |
| Tm | 60.108 | 60.036 | End: 475 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
|---|
| Sequence | CGCTTGCGCACCAAATGATA | GTTAGGGGTCTTTCGGCTCC | Start: 82 |
| Tm | 59.901 | 60.108 | End: 218 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | CGCTTGCGCACCAAATGATA | GGAGTTAGGGGTCTTTCGGC | Start: 82 |
| Tm | 59.901 | 60.108 | End: 221 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | GCTCCTGCAGTCTGGTCATT | TAGAGCAATCGAAGCCACGG | Start: 456 |
| Tm | 60.036 | 60.179 | End: 567 |
| Size | 20 | 20 | 112 |
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