Primer Details for TRINITY_DN65486_c7_g1_i1 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTCTGCCGTGCTAAGGAAGA | TGTCTCAGGCGATATTCTTTAGATGT | Start: 13 |
| Tm | 59.467 | 59.959 | End: 221 |
| Size | 20 | 26 | 209 |
| Primer 2 details |
|---|
| Sequence | TCGAGAGGTGCCAAATCTCC | TGTCTCAGGCGATATTCTTTAGATGT | Start: 48 |
| Tm | 59.463 | 59.959 | End: 221 |
| Size | 20 | 26 | 174 |
| Primer 3 details |
|---|
| Sequence | CGAGAGGTGCCAAATCTCCT | TGTCTCAGGCGATATTCTTTAGATGT | Start: 49 |
| Tm | 59.461 | 59.959 | End: 221 |
| Size | 20 | 26 | 173 |
| Primer 4 details |
|---|
| Sequence | GTGCTAAGGAAGAACGCCGT | TGTCTCAGGCGATATTCTTTAGATGT | Start: 20 |
| Tm | 60.67 | 59.959 | End: 221 |
| Size | 20 | 26 | 202 |
| Primer 5 details |
|---|
| Sequence | AACAGCTCTGCCGTGCTAAG | TGTCTCAGGCGATATTCTTTAGATGT | Start: 8 |
| Tm | 60.672 | 59.959 | End: 221 |
| Size | 20 | 26 | 214 |
