ssr_primer
Primer Details for TRINITY_DN65477_c2_g1_i9 |
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| (A)10tattgcataaagatgaccagtggcttaggttatgggaatagaaaacagaacatcaaa(T)13(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATACGAGCGCTCCATGTC | CTCTCGCCGACTGGATCATC | Start: 1985 |
| Tm | 59.969 | 60.04 | End: 2138 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
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| Sequence | AGCCGATCCGCTCTTTTTCA | ATTCTACTGCACCGTGCCAA | Start: 1235 |
| Tm | 60.036 | 59.964 | End: 1417 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
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| Sequence | CTGGTAGTGATGGCTCGCAT | GACATGGAGCGCTCGTATGA | Start: 1725 |
| Tm | 59.894 | 59.969 | End: 2004 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
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| Sequence | ATGGTTTGAGCGTGGTGAGT | GACATGGAGCGCTCGTATGA | Start: 1813 |
| Tm | 59.891 | 59.969 | End: 2004 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
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| Sequence | AGCCGATCCGCTCTTTTTCA | GATCCGAGGAGAGTTGGCAG | Start: 1235 |
| Tm | 60.036 | 59.896 | End: 1476 |
| Size | 20 | 20 | 242 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATACGAGCGCTCCATGTC | CTCTCGCCGACTGGATCATC | Start: 1985 |
| Tm | 59.969 | 60.04 | End: 2138 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | AGCCGATCCGCTCTTTTTCA | ATTCTACTGCACCGTGCCAA | Start: 1235 |
| Tm | 60.036 | 59.964 | End: 1417 |
| Size | 20 | 20 | 183 |
| Primer 3 details |
|---|
| Sequence | CTGGTAGTGATGGCTCGCAT | GACATGGAGCGCTCGTATGA | Start: 1725 |
| Tm | 59.894 | 59.969 | End: 2004 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
|---|
| Sequence | ATGGTTTGAGCGTGGTGAGT | GACATGGAGCGCTCGTATGA | Start: 1813 |
| Tm | 59.891 | 59.969 | End: 2004 |
| Size | 20 | 20 | 192 |
| Primer 5 details |
|---|
| Sequence | AGCCGATCCGCTCTTTTTCA | GATCCGAGGAGAGTTGGCAG | Start: 1235 |
| Tm | 60.036 | 59.896 | End: 1476 |
| Size | 20 | 20 | 242 |
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