ssr_primer
Primer Details for TRINITY_DN65471_c4_g1_i4 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGCGTACCTTCTGGAC | TACAGGAAGACCGAACGCAC | Start: 560 |
| Tm | 59.967 | 60.039 | End: 750 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | GTTCATTGGCAGCTTGGAGC | GTCCAGAAGGTACGCACACA | Start: 369 |
| Tm | 60.109 | 59.967 | End: 579 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GTGCGTACCTTCTGGACCTC | TACAGGAAGACCGAACGCAC | Start: 563 |
| Tm | 60.109 | 60.039 | End: 750 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | GGCCGCGTACTAGTGATCTC | TGATGAAGCCTTTTGCCCCT | Start: 2629 |
| Tm | 60.04 | 59.885 | End: 2876 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | TGTGTGCGTACCTTCTGGAC | TGTCAAGCCATGCTGGGAAA | Start: 560 |
| Tm | 59.967 | 60.179 | End: 806 |
| Size | 20 | 20 | 247 |
(C)14tcaagtgaaaaaaaagaaagaaaatcagatggaatgctaaaatttgtaagattcaaagaagagggctcacattt(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGCGTACCTTCTGGAC | TACAGGAAGACCGAACGCAC | Start: 560 |
| Tm | 59.967 | 60.039 | End: 750 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | GTTCATTGGCAGCTTGGAGC | GTCCAGAAGGTACGCACACA | Start: 369 |
| Tm | 60.109 | 59.967 | End: 579 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GTGCGTACCTTCTGGACCTC | TACAGGAAGACCGAACGCAC | Start: 563 |
| Tm | 60.109 | 60.039 | End: 750 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | GGCCGCGTACTAGTGATCTC | TGATGAAGCCTTTTGCCCCT | Start: 2629 |
| Tm | 60.04 | 59.885 | End: 2876 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | TGTGTGCGTACCTTCTGGAC | TGTCAAGCCATGCTGGGAAA | Start: 560 |
| Tm | 59.967 | 60.179 | End: 806 |
| Size | 20 | 20 | 247 |
(T)33: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTGCGTACCTTCTGGAC | TACAGGAAGACCGAACGCAC | Start: 560 |
| Tm | 59.967 | 60.039 | End: 750 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
|---|
| Sequence | GTTCATTGGCAGCTTGGAGC | GTCCAGAAGGTACGCACACA | Start: 369 |
| Tm | 60.109 | 59.967 | End: 579 |
| Size | 20 | 20 | 211 |
| Primer 3 details |
|---|
| Sequence | GTGCGTACCTTCTGGACCTC | TACAGGAAGACCGAACGCAC | Start: 563 |
| Tm | 60.109 | 60.039 | End: 750 |
| Size | 20 | 20 | 188 |
| Primer 4 details |
|---|
| Sequence | GGCCGCGTACTAGTGATCTC | TGATGAAGCCTTTTGCCCCT | Start: 2629 |
| Tm | 60.04 | 59.885 | End: 2876 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
|---|
| Sequence | TGTGTGCGTACCTTCTGGAC | TGTCAAGCCATGCTGGGAAA | Start: 560 |
| Tm | 59.967 | 60.179 | End: 806 |
| Size | 20 | 20 | 247 |
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