Primer Details for TRINITY_DN65404_c3_g1_i1 | |||
|---|---|---|---|
| (T)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGCGCCTCTTGGATTTTTA | ACCTTTTCCAAGCTATTACATCGA | Start: 36 |
| Tm | 59.55 | 58.259 | End: 144 |
| Size | 20 | 24 | 109 |
| Primer 2 details | |||
| Sequence | CAAAGATTGCGCGCCTCTTG | ACCTTTTCCAAGCTATTACATCGA | Start: 28 |
| Tm | 60.796 | 58.259 | End: 144 |
| Size | 20 | 24 | 117 |
| Primer 3 details | |||
| Sequence | GCGCGCCTCTTGGATTTTTA | AACCTTTTCCAAGCTATTACATCGA | Start: 36 |
| Tm | 59.55 | 58.822 | End: 145 |
| Size | 20 | 25 | 110 |
| Primer 4 details | |||
| Sequence | AAAACCAAAGATTGCGCGCC | ACCTTTTCCAAGCTATTACATCGA | Start: 23 |
| Tm | 60.944 | 58.259 | End: 144 |
| Size | 20 | 24 | 122 |
| Primer 5 details | |||
| Sequence | GCGCGCCTCTTGGATTTTTAT | ACCTTTTCCAAGCTATTACATCGA | Start: 36 |
| Tm | 59.934 | 58.259 | End: 144 |
| Size | 21 | 24 | 109 |
