ssr_primer
Primer Details for TRINITY_DN65375_c2_g1_i2 |
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| (T)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTGGTGTTCAACGAACGTGG | CACAAGTGCCCGAGGTAGTT | Start: 157 |
| Tm | 59.973 | 59.965 | End: 433 |
| Size | 20 | 20 | 277 |
| Primer 2 details |
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| Sequence | GTGGTGTTCAACGAACGTGG | CCCGAGGTAGTTGCCAAGTT | Start: 157 |
| Tm | 59.973 | 59.964 | End: 425 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
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| Sequence | TGCTGGGGAGAGGTTAGGTT | CACAAGTGCCCGAGGTAGTT | Start: 182 |
| Tm | 60.179 | 59.965 | End: 433 |
| Size | 20 | 20 | 252 |
| Primer 4 details |
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| Sequence | TGCTGGGGAGAGGTTAGGTT | CCCGAGGTAGTTGCCAAGTT | Start: 182 |
| Tm | 60.179 | 59.964 | End: 425 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
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| Sequence | GCTGGGGAGAGGTTAGGTTG | CACAAGTGCCCGAGGTAGTT | Start: 183 |
| Tm | 59.747 | 59.965 | End: 433 |
| Size | 20 | 20 | 251 |
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