ssr_primer
Primer Details for TRINITY_DN65374_c0_g1_i1 |
|---|
| (CTT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGCTTTGTTTGGACCGTG | AGGGCTGCAAGGAGATTGAC | Start: 13 |
| Tm | 59.969 | 60.035 | End: 190 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | CTTGGCAGGGGTGATTCCAT | AACAGTCACAGCTGAGACGG | Start: 591 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TTGACAATTCTCCCCCACCG | CAACACAGTGACAGTTGCCG | Start: 1793 |
| Tm | 59.962 | 59.972 | End: 1947 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GGCAGGGGTGATTCCATCAA | AACAGTCACAGCTGAGACGG | Start: 594 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | TTGGCAGGGGTGATTCCATC | AACAGTCACAGCTGAGACGG | Start: 592 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 222 |
(TTC)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTGCTTTGTTTGGACCGTG | AGGGCTGCAAGGAGATTGAC | Start: 13 |
| Tm | 59.969 | 60.035 | End: 190 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | CTTGGCAGGGGTGATTCCAT | AACAGTCACAGCTGAGACGG | Start: 591 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | TTGACAATTCTCCCCCACCG | CAACACAGTGACAGTTGCCG | Start: 1793 |
| Tm | 59.962 | 59.972 | End: 1947 |
| Size | 20 | 20 | 155 |
| Primer 4 details |
|---|
| Sequence | GGCAGGGGTGATTCCATCAA | AACAGTCACAGCTGAGACGG | Start: 594 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 220 |
| Primer 5 details |
|---|
| Sequence | TTGGCAGGGGTGATTCCATC | AACAGTCACAGCTGAGACGG | Start: 592 |
| Tm | 60.033 | 59.967 | End: 813 |
| Size | 20 | 20 | 222 |
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