ssr_primer
Primer Details for TRINITY_DN65307_c3_g1_i2 |
|---|
| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCACGCACCGCTTTCAAAC | ACGTTCGGATCCACCAGTTC | Start: 413 |
| Tm | 60.04 | 60.038 | End: 513 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | ATCACGCACCGCTTTCAAAC | GACGTTCGGATCCACCAGTT | Start: 413 |
| Tm | 60.04 | 60.038 | End: 514 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | GTCAGGGAAGTTGCTCCACA | CCCACTGAACAGACTCGACC | Start: 1742 |
| Tm | 59.891 | 60.038 | End: 1846 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
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| Sequence | CAGGGAAGTTGCTCCACAGT | CCCACTGAACAGACTCGACC | Start: 1744 |
| Tm | 59.891 | 60.038 | End: 1846 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
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| Sequence | AAGGTCCAGCAGCAATCCAA | GAGGAGCAGAAGCATGGGTT | Start: 2531 |
| Tm | 59.887 | 60.035 | End: 2785 |
| Size | 20 | 20 | 255 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCACGCACCGCTTTCAAAC | ACGTTCGGATCCACCAGTTC | Start: 413 |
| Tm | 60.04 | 60.038 | End: 513 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | ATCACGCACCGCTTTCAAAC | GACGTTCGGATCCACCAGTT | Start: 413 |
| Tm | 60.04 | 60.038 | End: 514 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | GTCAGGGAAGTTGCTCCACA | CCCACTGAACAGACTCGACC | Start: 1742 |
| Tm | 59.891 | 60.038 | End: 1846 |
| Size | 20 | 20 | 105 |
| Primer 4 details |
|---|
| Sequence | CAGGGAAGTTGCTCCACAGT | CCCACTGAACAGACTCGACC | Start: 1744 |
| Tm | 59.891 | 60.038 | End: 1846 |
| Size | 20 | 20 | 103 |
| Primer 5 details |
|---|
| Sequence | AAGGTCCAGCAGCAATCCAA | GAGGAGCAGAAGCATGGGTT | Start: 2531 |
| Tm | 59.887 | 60.035 | End: 2785 |
| Size | 20 | 20 | 255 |
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