ssr_primer
Primer Details for TRINITY_DN65281_c10_g1_i5 |
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| (A)22: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGATCAAGGGCGCATGGTA | CGTTTGTATGTGCCCCCTCT | Start: 9 |
| Tm | 59.893 | 60.035 | End: 265 |
| Size | 20 | 20 | 257 |
| Primer 2 details |
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| Sequence | GAGATCAAGGGCGCATGGTA | TCGTTTGTATGTGCCCCCTC | Start: 9 |
| Tm | 59.893 | 60.036 | End: 266 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
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| Sequence | GACGCTGCCAAATGAACCTC | CCGTTGGAACCTACAGCCAT | Start: 416 |
| Tm | 59.831 | 60.035 | End: 565 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
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| Sequence | AGAGGGGGCACATACAAACG | GAGGTTCATTTGGCAGCGTC | Start: 246 |
| Tm | 60.035 | 59.831 | End: 435 |
| Size | 20 | 20 | 190 |
| Primer 5 details |
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| Sequence | GACGCTGCCAAATGAACCTC | TTCCGTTGGAACCTACAGCC | Start: 416 |
| Tm | 59.831 | 59.964 | End: 567 |
| Size | 20 | 20 | 152 |
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