ssr_primer
Primer Details for TRINITY_DN65238_c7_g3_i1 |
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| (A)45: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGGGGAAGGGAGGGTG | TAAGCGGGAACAGCGAAGAG | Start: 538 |
| Tm | 59.81 | 60.109 | End: 812 |
| Size | 20 | 20 | 275 |
| Primer 2 details |
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| Sequence | TTTTTGGGGAAGGGAGGGTG | ACACCGGATTACCATCAGCC | Start: 538 |
| Tm | 59.81 | 59.82 | End: 770 |
| Size | 20 | 20 | 233 |
| Primer 3 details |
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| Sequence | TTTTTGGGGAAGGGAGGGTG | TGCGAACACCGGATTACCAT | Start: 538 |
| Tm | 59.81 | 59.749 | End: 775 |
| Size | 20 | 20 | 238 |
| Primer 4 details |
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| Sequence | TTTTTGGGGAAGGGAGGGTG | TTAAGCGGGAACAGCGAAGA | Start: 538 |
| Tm | 59.81 | 59.681 | End: 813 |
| Size | 20 | 20 | 276 |
| Primer 5 details |
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| Sequence | TTGGGGAAGGGAGGGTGATT | TAAGCGGGAACAGCGAAGAG | Start: 541 |
| Tm | 60.48 | 60.109 | End: 812 |
| Size | 20 | 20 | 272 |
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