Primer Details for TRINITY_DN65205_c0_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGTCGAACTCTACGAGGTT | AGCTTCTAGTTAGACCATTTGATCCA | Start: 121 |
| Tm | 59.832 | 59.843 | End: 300 |
| Size | 20 | 26 | 180 |
| Primer 2 details |
|---|
| Sequence | CAGCGTCGAACTCTACGAGG | AGCTTCTAGTTAGACCATTTGATCCA | Start: 119 |
| Tm | 60.248 | 59.843 | End: 300 |
| Size | 20 | 26 | 182 |
| Primer 3 details |
|---|
| Sequence | GCGTCGAACTCTACGAGGTT | GCTTCTAGTTAGACCATTTGATCCAC | Start: 121 |
| Tm | 59.832 | 59.741 | End: 299 |
| Size | 20 | 26 | 179 |
| Primer 4 details |
|---|
| Sequence | CAGCGTCGAACTCTACGAGG | GCTTCTAGTTAGACCATTTGATCCAC | Start: 119 |
| Tm | 60.248 | 59.741 | End: 299 |
| Size | 20 | 26 | 181 |
| Primer 5 details |
|---|
| Sequence | GCGTCGAACTCTACGAGGTT | GCTTCTAGTTAGACCATTTGATCCA | Start: 121 |
| Tm | 59.832 | 58.651 | End: 299 |
| Size | 20 | 25 | 179 |
(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCGTCGAACTCTACGAGGTT | AGCTTCTAGTTAGACCATTTGATCCA | Start: 121 |
| Tm | 59.832 | 59.843 | End: 300 |
| Size | 20 | 26 | 180 |
| Primer 2 details |
|---|
| Sequence | CAGCGTCGAACTCTACGAGG | AGCTTCTAGTTAGACCATTTGATCCA | Start: 119 |
| Tm | 60.248 | 59.843 | End: 300 |
| Size | 20 | 26 | 182 |
| Primer 3 details |
|---|
| Sequence | GCGTCGAACTCTACGAGGTT | GCTTCTAGTTAGACCATTTGATCCAC | Start: 121 |
| Tm | 59.832 | 59.741 | End: 299 |
| Size | 20 | 26 | 179 |
| Primer 4 details |
|---|
| Sequence | CAGCGTCGAACTCTACGAGG | GCTTCTAGTTAGACCATTTGATCCAC | Start: 119 |
| Tm | 60.248 | 59.741 | End: 299 |
| Size | 20 | 26 | 181 |
| Primer 5 details |
|---|
| Sequence | GCGTCGAACTCTACGAGGTT | GCTTCTAGTTAGACCATTTGATCCA | Start: 121 |
| Tm | 59.832 | 58.651 | End: 299 |
| Size | 20 | 25 | 179 |
