ssr_primer
Primer Details for TRINITY_DN65198_c5_g4_i1 |
|---|
| (T)44: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCCCTCCCCACGAAAAA | CGATTAAAGGCCCCCTCTCC | Start: 737 |
| Tm | 59.888 | 59.891 | End: 1010 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | AAATTTCTGACCCCGCCACT | CGATTAAAGGCCCCCTCTCC | Start: 754 |
| Tm | 59.888 | 59.891 | End: 1010 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | AAGGCTGGCGTAGAATCCTG | GGAGGGGAAGGATCTACGGT | Start: 561 |
| Tm | 59.821 | 60.106 | End: 746 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCCCGGATTAACGCCTAGTT | GGAGGGGAAGGATCTACGGT | Start: 531 |
| Tm | 60.179 | 60.106 | End: 746 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TGAACCAAGTCACAGACGGG | CAGGATTCTACGCCAGCCTT | Start: 436 |
| Tm | 59.894 | 59.821 | End: 580 |
| Size | 20 | 20 | 145 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTCCCCTCCCCACGAAAAA | CGATTAAAGGCCCCCTCTCC | Start: 737 |
| Tm | 59.888 | 59.891 | End: 1010 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | AAATTTCTGACCCCGCCACT | CGATTAAAGGCCCCCTCTCC | Start: 754 |
| Tm | 59.888 | 59.891 | End: 1010 |
| Size | 20 | 20 | 257 |
| Primer 3 details |
|---|
| Sequence | AAGGCTGGCGTAGAATCCTG | GGAGGGGAAGGATCTACGGT | Start: 561 |
| Tm | 59.821 | 60.106 | End: 746 |
| Size | 20 | 20 | 186 |
| Primer 4 details |
|---|
| Sequence | GCCCGGATTAACGCCTAGTT | GGAGGGGAAGGATCTACGGT | Start: 531 |
| Tm | 60.179 | 60.106 | End: 746 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TGAACCAAGTCACAGACGGG | CAGGATTCTACGCCAGCCTT | Start: 436 |
| Tm | 59.894 | 59.821 | End: 580 |
| Size | 20 | 20 | 145 |
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