ssr_primer
Primer Details for TRINITY_DN65188_c4_g3_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCAGAGTGGTGACGACAA | CTGACAAGACTGCTGGGGAG | Start: 274 |
| Tm | 59.968 | 60.037 | End: 526 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | CTCCCCAGCAGTCTTGTCAG | TATTCCATGGATGCGGCTCC | Start: 507 |
| Tm | 60.037 | 59.963 | End: 607 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
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| Sequence | GCCAGATCCGCTAACGAAGA | TTGTCGTCACCACTCTGCTC | Start: 151 |
| Tm | 59.898 | 59.968 | End: 293 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
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| Sequence | CCAACTTGCCAGATCCGCTA | TTGTCGTCACCACTCTGCTC | Start: 144 |
| Tm | 60.108 | 59.968 | End: 293 |
| Size | 20 | 20 | 150 |
| Primer 5 details |
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| Sequence | GAGAGCAGAGTGGTGACGAC | CTGACAAGACTGCTGGGGAG | Start: 272 |
| Tm | 60.11 | 60.037 | End: 526 |
| Size | 20 | 20 | 255 |
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