Primer Details for TRINITY_DN65183_c6_g1_i1 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGTACAATGGAGAAAATTCGCC | TGGAGTTCAATCTTATAGCCTGTCA | Start: 37 |
| Tm | 59.627 | 59.575 | End: 235 |
| Size | 23 | 25 | 199 |
| Primer 2 details |
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| Sequence | TGTGGGGATTCCTTTGAAACT | TGGAGTTCAATCTTATAGCCTGTCA | Start: 1 |
| Tm | 57.389 | 59.575 | End: 235 |
| Size | 21 | 25 | 235 |
| Primer 3 details |
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| Sequence | CGTACAATGGAGAAAATTCGCC | TGGAGTTCAATCTTATAGCCTGTCA | Start: 38 |
| Tm | 58.319 | 59.575 | End: 235 |
| Size | 22 | 25 | 198 |
| Primer 4 details |
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| Sequence | TCGTACAATGGAGAAAATTCGCC | GGAGTTCAATCTTATAGCCTGTCA | Start: 37 |
| Tm | 59.627 | 58.081 | End: 234 |
| Size | 23 | 24 | 198 |
| Primer 5 details |
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| Sequence | TCGTACAATGGAGAAAATTCGCC | TGGAGTTCAATCTTATAGCCTGTC | Start: 37 |
| Tm | 59.627 | 58.081 | End: 235 |
| Size | 23 | 24 | 199 |
