ssr_primer
Primer Details for TRINITY_DN65171_c4_g5_i1 |
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| (A)11gaacagaaaaaaagaaagaaaaaaagaggaagagaaaagaaaaaaaagaaataagagaagaagaaaaaaaag(A)20ggaaagaac(A)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACGCTGCATACAATCACGC | TGTGCCTCCCAATGCATCTT | Start: 457 |
| Tm | 59.903 | 59.96 | End: 561 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | AACGCTGCATACAATCACGC | TTGTGCCTCCCAATGCATCT | Start: 457 |
| Tm | 59.903 | 59.96 | End: 562 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
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| Sequence | AACGCTGCATACAATCACGC | GTTGTGCCTCCCAATGCATC | Start: 457 |
| Tm | 59.903 | 59.826 | End: 563 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
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| Sequence | AACGCTGCATACAATCACGC | ACACATGGGAGTTGTGCCTC | Start: 457 |
| Tm | 59.903 | 60.251 | End: 573 |
| Size | 20 | 20 | 117 |
| Primer 5 details |
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| Sequence | AACGCTGCATACAATCACGC | GACACATGGGAGTTGTGCCT | Start: 457 |
| Tm | 59.903 | 60.251 | End: 574 |
| Size | 20 | 20 | 118 |
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