ssr_primer
Primer Details for TRINITY_DN65130_c1_g5_i1 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCAGACACCCGCAAACG | ACAGGTTCCTCAGTGAAATGGA | Start: 55 |
| Tm | 59.621 | 59.289 | End: 229 |
| Size | 20 | 22 | 175 |
| Primer 2 details |
|---|
| Sequence | AGACACCCGCAAACGAGTTA | ACAGGTTCCTCAGTGAAATGGA | Start: 60 |
| Tm | 59.61 | 59.289 | End: 229 |
| Size | 20 | 22 | 170 |
| Primer 3 details |
|---|
| Sequence | TTTCAGACACCCGCAAACGA | ACAGGTTCCTCAGTGAAATGGA | Start: 56 |
| Tm | 60.462 | 59.289 | End: 229 |
| Size | 20 | 22 | 174 |
| Primer 4 details |
|---|
| Sequence | TTTTCAGACACCCGCAAACG | GGTTCCTCAGTGAAATGGAATGC | Start: 55 |
| Tm | 59.621 | 60.119 | End: 226 |
| Size | 20 | 23 | 172 |
| Primer 5 details |
|---|
| Sequence | AGACACCCGCAAACGAGTTA | GGTTCCTCAGTGAAATGGAATGC | Start: 60 |
| Tm | 59.61 | 60.119 | End: 226 |
| Size | 20 | 23 | 167 |
(T)13g(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTCAGACACCCGCAAACG | ACAGGTTCCTCAGTGAAATGGA | Start: 55 |
| Tm | 59.621 | 59.289 | End: 229 |
| Size | 20 | 22 | 175 |
| Primer 2 details |
|---|
| Sequence | AGACACCCGCAAACGAGTTA | ACAGGTTCCTCAGTGAAATGGA | Start: 60 |
| Tm | 59.61 | 59.289 | End: 229 |
| Size | 20 | 22 | 170 |
| Primer 3 details |
|---|
| Sequence | TTTCAGACACCCGCAAACGA | ACAGGTTCCTCAGTGAAATGGA | Start: 56 |
| Tm | 60.462 | 59.289 | End: 229 |
| Size | 20 | 22 | 174 |
| Primer 4 details |
|---|
| Sequence | TTTTCAGACACCCGCAAACG | GGTTCCTCAGTGAAATGGAATGC | Start: 55 |
| Tm | 59.621 | 60.119 | End: 226 |
| Size | 20 | 23 | 172 |
| Primer 5 details |
|---|
| Sequence | AGACACCCGCAAACGAGTTA | GGTTCCTCAGTGAAATGGAATGC | Start: 60 |
| Tm | 59.61 | 60.119 | End: 226 |
| Size | 20 | 23 | 167 |
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