ssr_primer
Primer Details for TRINITY_DN65116_c4_g1_i5 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATCGGTCGCAAACTTCTCGT | ACTCACCACAGTGCAAACCA | Start: 2017 |
| Tm | 60.039 | 60.035 | End: 2147 |
| Size | 20 | 20 | 131 |
| Primer 2 details |
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| Sequence | TTTGAGCCAGCTTCAGGAGG | ACGAGAAGTTTGCGACCGAT | Start: 1852 |
| Tm | 59.963 | 60.039 | End: 2036 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
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| Sequence | CAGGGTATCGGTCGCAAACT | ACTCACCACAGTGCAAACCA | Start: 2011 |
| Tm | 60.108 | 60.035 | End: 2147 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | TTTGAGCCAGCTTCAGGAGG | AGTTTGCGACCGATACCCTG | Start: 1852 |
| Tm | 59.963 | 60.108 | End: 2030 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
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| Sequence | ATCGGTCGCAAACTTCTCGT | ATGCTGCCAAAAGGACTCCA | Start: 2017 |
| Tm | 60.039 | 59.887 | End: 2168 |
| Size | 20 | 20 | 152 |
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