Primer Details for TRINITY_DN65050_c3_g1_i1 | |||
|---|---|---|---|
| (T)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGACTCTTAAAAACATCGGCA | ACATTGACAACCTTGATTCGCA | Start: 11 |
| Tm | 58.363 | 59.116 | End: 156 |
| Size | 23 | 22 | 146 |
| Primer 2 details | |||
| Sequence | TCAGACTCTTAAAAACATCGGCA | TGACAACCTTGATTCGCAATCA | Start: 11 |
| Tm | 58.363 | 58.855 | End: 152 |
| Size | 23 | 22 | 142 |
| Primer 3 details | |||
| Sequence | GTTCAGACTCTTAAAAACATCGGCA | ACATTGACAACCTTGATTCGCA | Start: 9 |
| Tm | 60.05 | 59.116 | End: 156 |
| Size | 25 | 22 | 148 |
| Primer 4 details | |||
| Sequence | TCAGACTCTTAAAAACATCGGCAA | ACATTGACAACCTTGATTCGCA | Start: 11 |
| Tm | 58.939 | 59.116 | End: 156 |
| Size | 24 | 22 | 146 |
| Primer 5 details | |||
| Sequence | TTCAGACTCTTAAAAACATCGGCA | ACATTGACAACCTTGATTCGCA | Start: 10 |
| Tm | 58.939 | 59.116 | End: 156 |
| Size | 24 | 22 | 147 |
