ssr_primer
Primer Details for TRINITY_DN65049_c2_g1_i21 |
|---|
| (A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGAAACTTTGCGCCGTTT | AACCAGGCATGAGGAATGGG | Start: 137 |
| Tm | 59.97 | 60.033 | End: 253 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CCAAGGGATGCAACTTTGGC | AAACGGCGCAAAGTTTCTCC | Start: 2 |
| Tm | 60.037 | 59.97 | End: 156 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | GGAGAAACTTTGCGCCGTTT | GGTGAACCAGGCATGAGGAA | Start: 137 |
| Tm | 59.97 | 59.962 | End: 257 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | CCCATTCCTCATGCCTGGTT | TGCACTTGTTCCAGCGGTAT | Start: 234 |
| Tm | 60.033 | 59.964 | End: 491 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | GGAGAAACTTTGCGCCGTTT | AGGAATGGGCAGGAGTGTTG | Start: 137 |
| Tm | 59.97 | 59.961 | End: 242 |
| Size | 20 | 20 | 106 |
(A)10gttttaacagaggtactcaataaaaagagcctcatttgtcattatttcttggcatggc(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAGAAACTTTGCGCCGTTT | AACCAGGCATGAGGAATGGG | Start: 137 |
| Tm | 59.97 | 60.033 | End: 253 |
| Size | 20 | 20 | 117 |
| Primer 2 details |
|---|
| Sequence | CCAAGGGATGCAACTTTGGC | AAACGGCGCAAAGTTTCTCC | Start: 2 |
| Tm | 60.037 | 59.97 | End: 156 |
| Size | 20 | 20 | 155 |
| Primer 3 details |
|---|
| Sequence | GGAGAAACTTTGCGCCGTTT | GGTGAACCAGGCATGAGGAA | Start: 137 |
| Tm | 59.97 | 59.962 | End: 257 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
|---|
| Sequence | CCCATTCCTCATGCCTGGTT | TGCACTTGTTCCAGCGGTAT | Start: 234 |
| Tm | 60.033 | 59.964 | End: 491 |
| Size | 20 | 20 | 258 |
| Primer 5 details |
|---|
| Sequence | GGAGAAACTTTGCGCCGTTT | AGGAATGGGCAGGAGTGTTG | Start: 137 |
| Tm | 59.97 | 59.961 | End: 242 |
| Size | 20 | 20 | 106 |
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