Primer Details for TRINITY_DN65029_c6_g4_i1 | |||
|---|---|---|---|
| (T)19(GA)6: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCGCCCAATACGCAGTT | AGTCACAAAACTCTCTCTCTCTCA | Start: 10 |
| Tm | 60.037 | 58.863 | End: 162 |
| Size | 20 | 24 | 153 |
| Primer 2 details | |||
| Sequence | ACTTCTTCGCCCAATACGCA | AGTCACAAAACTCTCTCTCTCTCA | Start: 7 |
| Tm | 60.037 | 58.863 | End: 162 |
| Size | 20 | 24 | 156 |
| Primer 3 details | |||
| Sequence | TCTTCGCCCAATACGCAGTT | GTCACAAAACTCTCTCTCTCTCA | Start: 10 |
| Tm | 60.037 | 57.554 | End: 161 |
| Size | 20 | 23 | 152 |
| Primer 4 details | |||
| Sequence | ACTTCTTCGCCCAATACGCA | GTCACAAAACTCTCTCTCTCTCA | Start: 7 |
| Tm | 60.037 | 57.554 | End: 161 |
| Size | 20 | 23 | 155 |
| Primer 5 details | |||
| Sequence | TCTTCGCCCAATACGCAGTT | AGTCACAAAACTCTCTCTCTCTCAA | Start: 10 |
| Tm | 60.037 | 59.404 | End: 162 |
| Size | 20 | 25 | 153 |
