Primer Details for TRINITY_DN65014_c1_g1_i1 |
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| (T)22g(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACCATCGGCAATGCATACA | ACACAAAACAAAACACACTTTTTCA | Start: 26 |
| Tm | 58.239 | 57.691 | End: 228 |
| Size | 20 | 25 | 203 |
| Primer 2 details |
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| Sequence | ACCATCGGCAATGCATACAT | ACACAAAACAAAACACACTTTTTCA | Start: 27 |
| Tm | 58.015 | 57.691 | End: 228 |
| Size | 20 | 25 | 202 |
| Primer 3 details |
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| Sequence | ACTTTAACCATCGGCAATGCA | ACACAAAACAAAACACACTTTTTCA | Start: 21 |
| Tm | 58.833 | 57.691 | End: 228 |
| Size | 21 | 25 | 208 |
| Primer 4 details |
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| Sequence | AACCATCGGCAATGCATACAT | ACACAAAACAAAACACACTTTTTCA | Start: 26 |
| Tm | 58.69 | 57.691 | End: 228 |
| Size | 21 | 25 | 203 |
| Primer 5 details |
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| Sequence | AACCATCGGCAATGCATACA | ACACAAAACAAAACACACTTTTTCAT | Start: 26 |
| Tm | 58.239 | 58.075 | End: 228 |
| Size | 20 | 26 | 203 |
