ssr_primer
Primer Details for TRINITY_DN65012_c2_g11_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGAGTGGGTTGTCCGTAACA | AACGTGGAAGGTGCGTGTAT | Start: 319 |
| Tm | 59.968 | 59.966 | End: 481 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
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| Sequence | GAACCAGATGGCGGAAGTGA | TGTTACGGACAACCCACTCG | Start: 202 |
| Tm | 60.036 | 59.968 | End: 338 |
| Size | 20 | 20 | 137 |
| Primer 3 details |
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| Sequence | GAACCAGATGGCGGAAGTGA | AACGTGGAAGGTGCGTGTAT | Start: 202 |
| Tm | 60.036 | 59.966 | End: 481 |
| Size | 20 | 20 | 280 |
| Primer 4 details |
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| Sequence | TCATACGGCGTTCTTCCTCG | TGTTACGGACAACCCACTCG | Start: 87 |
| Tm | 59.9 | 59.968 | End: 338 |
| Size | 20 | 20 | 252 |
| Primer 5 details |
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| Sequence | TCATACGGCGTTCTTCCTCG | TCACTTCCGCCATCTGGTTC | Start: 87 |
| Tm | 59.9 | 60.036 | End: 221 |
| Size | 20 | 20 | 135 |
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