ssr_primer
Primer Details for TRINITY_DN65011_c5_g1_i2 |
|---|
| (C)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTGCGAAAGTGCGATAAG | TTACACTTCCGCGCATGAGT | Start: 638 |
| Tm | 59.973 | 60.038 | End: 820 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | GAGCCTCGTACTCCCGATTG | CCAGCACCCAACCCAAATTG | Start: 1710 |
| Tm | 59.969 | 59.965 | End: 1857 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | CTTATCGCACTTTCGCAGCC | Start: 546 |
| Tm | 59.899 | 59.973 | End: 657 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | GGCTTATCGCACTTTCGCAG | Start: 546 |
| Tm | 59.899 | 59.973 | End: 659 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | GACCCTTTCTGCACCCTCTC | CGATGACTTCCCCTCGACAG | Start: 335 |
| Tm | 60.036 | 59.899 | End: 565 |
| Size | 20 | 20 | 231 |
(T)10ctctttatttttggttttgtctgtttttttttagtttct(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTGCGAAAGTGCGATAAG | TTACACTTCCGCGCATGAGT | Start: 638 |
| Tm | 59.973 | 60.038 | End: 820 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | GAGCCTCGTACTCCCGATTG | CCAGCACCCAACCCAAATTG | Start: 1710 |
| Tm | 59.969 | 59.965 | End: 1857 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | CTTATCGCACTTTCGCAGCC | Start: 546 |
| Tm | 59.899 | 59.973 | End: 657 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | GGCTTATCGCACTTTCGCAG | Start: 546 |
| Tm | 59.899 | 59.973 | End: 659 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | GACCCTTTCTGCACCCTCTC | CGATGACTTCCCCTCGACAG | Start: 335 |
| Tm | 60.036 | 59.899 | End: 565 |
| Size | 20 | 20 | 231 |
(G)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCTGCGAAAGTGCGATAAG | TTACACTTCCGCGCATGAGT | Start: 638 |
| Tm | 59.973 | 60.038 | End: 820 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
|---|
| Sequence | GAGCCTCGTACTCCCGATTG | CCAGCACCCAACCCAAATTG | Start: 1710 |
| Tm | 59.969 | 59.965 | End: 1857 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | CTTATCGCACTTTCGCAGCC | Start: 546 |
| Tm | 59.899 | 59.973 | End: 657 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
|---|
| Sequence | CTGTCGAGGGGAAGTCATCG | GGCTTATCGCACTTTCGCAG | Start: 546 |
| Tm | 59.899 | 59.973 | End: 659 |
| Size | 20 | 20 | 114 |
| Primer 5 details |
|---|
| Sequence | GACCCTTTCTGCACCCTCTC | CGATGACTTCCCCTCGACAG | Start: 335 |
| Tm | 60.036 | 59.899 | End: 565 |
| Size | 20 | 20 | 231 |
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