ssr_primer
Primer Details for TRINITY_DN64991_c4_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTGGGTAATGTATCCGCCCC | TTAGTGATTGGGCCCTGCAG | Start: 318 |
| Tm | 59.964 | 60.034 | End: 493 |
| Size | 20 | 20 | 176 |
| Primer 2 details |
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| Sequence | CTGGGTAATGTATCCGCCCC | TTTTAAGGAAGCGGAGGCGT | Start: 318 |
| Tm | 59.964 | 59.964 | End: 438 |
| Size | 20 | 20 | 121 |
| Primer 3 details |
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| Sequence | CTGGGTAATGTATCCGCCCC | CAGCGACCAAGGAGAGTACC | Start: 318 |
| Tm | 59.964 | 59.826 | End: 476 |
| Size | 20 | 20 | 159 |
| Primer 4 details |
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| Sequence | GCGGTTCCAGGATCACTCTC | TTAGTGATTGGGCCCTGCAG | Start: 246 |
| Tm | 60.179 | 60.034 | End: 493 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
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| Sequence | GCGGTTCCAGGATCACTCTC | TTTTAAGGAAGCGGAGGCGT | Start: 246 |
| Tm | 60.179 | 59.964 | End: 438 |
| Size | 20 | 20 | 193 |
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