ssr_primer
Primer Details for TRINITY_DN64964_c1_g3_i9 |
|---|
| (C)10attccactcgccgtggaaatgctgaccatccagggctacgtttaaagaaatagcacgtgcacccagaacaacacgccggtttagcaaacgaaaaaaaaa(G)10aaataaccaacacacaaagacagcg(A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCGATACACAGGACTCCG | CGTTGCGCTTTACATACGGG | Start: 1451 |
| Tm | 59.97 | 59.974 | End: 1612 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | CGAGCGATACACAGGACTCC | CGTTGCGCTTTACATACGGG | Start: 1450 |
| Tm | 59.97 | 59.974 | End: 1612 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GTATTCATCGGACGAGCGGT | TTTGCTAAACCGGCGTGTTG | Start: 389 |
| Tm | 59.97 | 59.97 | End: 611 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | GTATTCATCGGACGAGCGGT | GTTTGCTAAACCGGCGTGTT | Start: 389 |
| Tm | 59.97 | 59.97 | End: 612 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AACGAGAGGGAACGACACAC | GGAGTCCTGTGTATCGCTCG | Start: 1308 |
| Tm | 59.968 | 59.97 | End: 1469 |
| Size | 20 | 20 | 162 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCGATACACAGGACTCCG | CGTTGCGCTTTACATACGGG | Start: 1451 |
| Tm | 59.97 | 59.974 | End: 1612 |
| Size | 20 | 20 | 162 |
| Primer 2 details |
|---|
| Sequence | CGAGCGATACACAGGACTCC | CGTTGCGCTTTACATACGGG | Start: 1450 |
| Tm | 59.97 | 59.974 | End: 1612 |
| Size | 20 | 20 | 163 |
| Primer 3 details |
|---|
| Sequence | GTATTCATCGGACGAGCGGT | TTTGCTAAACCGGCGTGTTG | Start: 389 |
| Tm | 59.97 | 59.97 | End: 611 |
| Size | 20 | 20 | 223 |
| Primer 4 details |
|---|
| Sequence | GTATTCATCGGACGAGCGGT | GTTTGCTAAACCGGCGTGTT | Start: 389 |
| Tm | 59.97 | 59.97 | End: 612 |
| Size | 20 | 20 | 224 |
| Primer 5 details |
|---|
| Sequence | AACGAGAGGGAACGACACAC | GGAGTCCTGTGTATCGCTCG | Start: 1308 |
| Tm | 59.968 | 59.97 | End: 1469 |
| Size | 20 | 20 | 162 |
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