ssr_primer
Primer Details for TRINITY_DN64878_c9_g3_i1 |
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| (T)14(G)14ccccccggcggggggccttttgggacaaaccttttttc(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCAAAACACTCACGAACCC | TCCTGCATTTTTGCAACGGT | Start: 34 |
| Tm | 59.97 | 59.25 | End: 191 |
| Size | 20 | 20 | 158 |
| Primer 2 details |
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| Sequence | GGCCAAAACACTCACGAACC | TCCTGCATTTTTGCAACGGT | Start: 33 |
| Tm | 59.97 | 59.25 | End: 191 |
| Size | 20 | 20 | 159 |
| Primer 3 details |
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| Sequence | GCCAAAACACTCACGAACCC | AGGTTTGTCCCAAAAGGCCC | Start: 34 |
| Tm | 59.97 | 60.763 | End: 270 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
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| Sequence | GGCCAAAACACTCACGAACC | AGGTTTGTCCCAAAAGGCCC | Start: 33 |
| Tm | 59.97 | 60.763 | End: 270 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
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| Sequence | AAACACTCACGAACCCCCTC | TCCTGCATTTTTGCAACGGT | Start: 38 |
| Tm | 59.892 | 59.25 | End: 191 |
| Size | 20 | 20 | 154 |
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