ssr_primer
Primer Details for TRINITY_DN64871_c9_g2_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGGCTGCGACTAAAGCTA | CACATCCCCACCTCCTCAAC | Start: 387 |
| Tm | 59.824 | 60.035 | End: 534 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GACTAAAGCTAGGGCCCACG | CACATCCCCACCTCCTCAAC | Start: 396 |
| Tm | 60.179 | 60.035 | End: 534 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | CGACTAAAGCTAGGGCCCAC | CACATCCCCACCTCCTCAAC | Start: 395 |
| Tm | 60.179 | 60.035 | End: 534 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | CCAGGCTGCGACTAAAGCTA | ATCCCCACCTCCTCAACAGA | Start: 387 |
| Tm | 59.824 | 59.881 | End: 531 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GACTAAAGCTAGGGCCCACG | ATCCCCACCTCCTCAACAGA | Start: 396 |
| Tm | 60.179 | 59.881 | End: 531 |
| Size | 20 | 20 | 136 |
(AAT)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGGCTGCGACTAAAGCTA | CACATCCCCACCTCCTCAAC | Start: 387 |
| Tm | 59.824 | 60.035 | End: 534 |
| Size | 20 | 20 | 148 |
| Primer 2 details |
|---|
| Sequence | GACTAAAGCTAGGGCCCACG | CACATCCCCACCTCCTCAAC | Start: 396 |
| Tm | 60.179 | 60.035 | End: 534 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | CGACTAAAGCTAGGGCCCAC | CACATCCCCACCTCCTCAAC | Start: 395 |
| Tm | 60.179 | 60.035 | End: 534 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | CCAGGCTGCGACTAAAGCTA | ATCCCCACCTCCTCAACAGA | Start: 387 |
| Tm | 59.824 | 59.881 | End: 531 |
| Size | 20 | 20 | 145 |
| Primer 5 details |
|---|
| Sequence | GACTAAAGCTAGGGCCCACG | ATCCCCACCTCCTCAACAGA | Start: 396 |
| Tm | 60.179 | 59.881 | End: 531 |
| Size | 20 | 20 | 136 |
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