ssr_primer
Primer Details for TRINITY_DN64861_c0_g1_i3 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCCTGCCTTTGCCTGAA | GGTGCTGAAAACGACGATGG | Start: 700 |
| Tm | 60.106 | 59.834 | End: 840 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | GCGGAATTACACCTCAAGCG | AGCTTTAGGTTGCTCTCGCA | Start: 1326 |
| Tm | 59.626 | 59.678 | End: 1546 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | TTCCCTGCCTTTGCCTGAAG | GGTGCTGAAAACGACGATGG | Start: 701 |
| Tm | 60.54 | 59.834 | End: 840 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | TAGTTTCCCTGCCTTTGCCT | GGTGCTGAAAACGACGATGG | Start: 697 |
| Tm | 59.223 | 59.834 | End: 840 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCTGCCTTTGCCTGAA | TGGTGCTGAAAACGACGATG | Start: 700 |
| Tm | 60.106 | 59.135 | End: 841 |
| Size | 20 | 20 | 142 |
(A)10ttgtttttacgag(A)52: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCCCTGCCTTTGCCTGAA | GGTGCTGAAAACGACGATGG | Start: 700 |
| Tm | 60.106 | 59.834 | End: 840 |
| Size | 20 | 20 | 141 |
| Primer 2 details |
|---|
| Sequence | GCGGAATTACACCTCAAGCG | AGCTTTAGGTTGCTCTCGCA | Start: 1326 |
| Tm | 59.626 | 59.678 | End: 1546 |
| Size | 20 | 20 | 221 |
| Primer 3 details |
|---|
| Sequence | TTCCCTGCCTTTGCCTGAAG | GGTGCTGAAAACGACGATGG | Start: 701 |
| Tm | 60.54 | 59.834 | End: 840 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | TAGTTTCCCTGCCTTTGCCT | GGTGCTGAAAACGACGATGG | Start: 697 |
| Tm | 59.223 | 59.834 | End: 840 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
|---|
| Sequence | TTTCCCTGCCTTTGCCTGAA | TGGTGCTGAAAACGACGATG | Start: 700 |
| Tm | 60.106 | 59.135 | End: 841 |
| Size | 20 | 20 | 142 |
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