ssr_primer
Primer Details for TRINITY_DN64808_c8_g1_i1 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTAGATGACAGCCCCCTCT | TCACCGTGCTTAAACCTGCA | Start: 57 |
| Tm | 60.105 | 60.179 | End: 198 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | AACTCACAAGTTGCCCACGA | TCACCGTGCTTAAACCTGCA | Start: 86 |
| Tm | 60.107 | 60.179 | End: 198 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | CTGCAGACCTCCTGGCATAC | ACGTATCTCGCATGCAGTGT | Start: 388 |
| Tm | 60.179 | 59.827 | End: 563 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
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| Sequence | TGCAGGTTTAAGCACGGTGA | GTATGCCAGGAGGTCTGCAG | Start: 179 |
| Tm | 60.179 | 60.179 | End: 407 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | CAAGTTGCCCACGAGGATGA | TCACCGTGCTTAAACCTGCA | Start: 92 |
| Tm | 60.322 | 60.179 | End: 198 |
| Size | 20 | 20 | 107 |
(A)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTAGATGACAGCCCCCTCT | TCACCGTGCTTAAACCTGCA | Start: 57 |
| Tm | 60.105 | 60.179 | End: 198 |
| Size | 20 | 20 | 142 |
| Primer 2 details |
|---|
| Sequence | AACTCACAAGTTGCCCACGA | TCACCGTGCTTAAACCTGCA | Start: 86 |
| Tm | 60.107 | 60.179 | End: 198 |
| Size | 20 | 20 | 113 |
| Primer 3 details |
|---|
| Sequence | CTGCAGACCTCCTGGCATAC | ACGTATCTCGCATGCAGTGT | Start: 388 |
| Tm | 60.179 | 59.827 | End: 563 |
| Size | 20 | 20 | 176 |
| Primer 4 details |
|---|
| Sequence | TGCAGGTTTAAGCACGGTGA | GTATGCCAGGAGGTCTGCAG | Start: 179 |
| Tm | 60.179 | 60.179 | End: 407 |
| Size | 20 | 20 | 229 |
| Primer 5 details |
|---|
| Sequence | CAAGTTGCCCACGAGGATGA | TCACCGTGCTTAAACCTGCA | Start: 92 |
| Tm | 60.322 | 60.179 | End: 198 |
| Size | 20 | 20 | 107 |
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