ssr_primer
Primer Details for TRINITY_DN64748_c5_g1_i5 |
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| (T)20aattgattgaatccatttctttcgacaatagcggatacctgctcaggttttacagatttttagtatgtcttctattgagttataccg(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCGCTTGCAAATTTCCGG | GTGACAGGACGTCTCTTGCA | Start: 1749 |
| Tm | 60.04 | 59.968 | End: 1932 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
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| Sequence | AATCAGTGGCCGTAGTTGGG | CCGGAAATTTGCAAGCGACA | Start: 1574 |
| Tm | 60.035 | 60.04 | End: 1768 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
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| Sequence | TTTGAAGGCGCTCCAAATGC | CCGGAAATTTGCAAGCGACA | Start: 1652 |
| Tm | 60.038 | 60.04 | End: 1768 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
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| Sequence | CCAGTCGCGTGATTCTCGTA | CCCAACTACGGCCACTGATT | Start: 1362 |
| Tm | 59.902 | 60.035 | End: 1593 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
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| Sequence | CGTGCAGTGAAATCAGTGGC | GCATTTGGAGCGCCTTCAAA | Start: 1564 |
| Tm | 60.11 | 60.038 | End: 1671 |
| Size | 20 | 20 | 108 |
(A)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCGCTTGCAAATTTCCGG | GTGACAGGACGTCTCTTGCA | Start: 1749 |
| Tm | 60.04 | 59.968 | End: 1932 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | AATCAGTGGCCGTAGTTGGG | CCGGAAATTTGCAAGCGACA | Start: 1574 |
| Tm | 60.035 | 60.04 | End: 1768 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | TTTGAAGGCGCTCCAAATGC | CCGGAAATTTGCAAGCGACA | Start: 1652 |
| Tm | 60.038 | 60.04 | End: 1768 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | CCAGTCGCGTGATTCTCGTA | CCCAACTACGGCCACTGATT | Start: 1362 |
| Tm | 59.902 | 60.035 | End: 1593 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CGTGCAGTGAAATCAGTGGC | GCATTTGGAGCGCCTTCAAA | Start: 1564 |
| Tm | 60.11 | 60.038 | End: 1671 |
| Size | 20 | 20 | 108 |
(A)13taaaaaaat(A)10t(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTCGCTTGCAAATTTCCGG | GTGACAGGACGTCTCTTGCA | Start: 1749 |
| Tm | 60.04 | 59.968 | End: 1932 |
| Size | 20 | 20 | 184 |
| Primer 2 details |
|---|
| Sequence | AATCAGTGGCCGTAGTTGGG | CCGGAAATTTGCAAGCGACA | Start: 1574 |
| Tm | 60.035 | 60.04 | End: 1768 |
| Size | 20 | 20 | 195 |
| Primer 3 details |
|---|
| Sequence | TTTGAAGGCGCTCCAAATGC | CCGGAAATTTGCAAGCGACA | Start: 1652 |
| Tm | 60.038 | 60.04 | End: 1768 |
| Size | 20 | 20 | 117 |
| Primer 4 details |
|---|
| Sequence | CCAGTCGCGTGATTCTCGTA | CCCAACTACGGCCACTGATT | Start: 1362 |
| Tm | 59.902 | 60.035 | End: 1593 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
|---|
| Sequence | CGTGCAGTGAAATCAGTGGC | GCATTTGGAGCGCCTTCAAA | Start: 1564 |
| Tm | 60.11 | 60.038 | End: 1671 |
| Size | 20 | 20 | 108 |
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