ssr_primer
Primer Details for TRINITY_DN64723_c5_g1_i3 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACAGGACCTGGGATCTT | TCATCTCGGTTTTCCACCCG | Start: 1758 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | TTCGACAGGACCTGGGATCT | TCATCTCGGTTTTCCACCCG | Start: 1757 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | CGCTCTTCCGATCTCGACAA | TGGTCCCAGTAAGGCGAGTA | Start: 44 |
| Tm | 59.901 | 59.96 | End: 298 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | TCTGTTTGCGTTCGACAGGA | TCATCTCGGTTTTCCACCCG | Start: 1747 |
| Tm | 59.897 | 60.037 | End: 1987 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AAAACGCGCCCTTTATGCTG | TTTGCCCAGAAATTTGGCCG | Start: 1579 |
| Tm | 60.109 | 59.965 | End: 1809 |
| Size | 20 | 20 | 231 |
(T)11aaatattcgttatgtaaggttaggttatttagctccttgatgatgtaatgattttcttttttttt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACAGGACCTGGGATCTT | TCATCTCGGTTTTCCACCCG | Start: 1758 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | TTCGACAGGACCTGGGATCT | TCATCTCGGTTTTCCACCCG | Start: 1757 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | CGCTCTTCCGATCTCGACAA | TGGTCCCAGTAAGGCGAGTA | Start: 44 |
| Tm | 59.901 | 59.96 | End: 298 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | TCTGTTTGCGTTCGACAGGA | TCATCTCGGTTTTCCACCCG | Start: 1747 |
| Tm | 59.897 | 60.037 | End: 1987 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AAAACGCGCCCTTTATGCTG | TTTGCCCAGAAATTTGGCCG | Start: 1579 |
| Tm | 60.109 | 59.965 | End: 1809 |
| Size | 20 | 20 | 231 |
(G)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGACAGGACCTGGGATCTT | TCATCTCGGTTTTCCACCCG | Start: 1758 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 230 |
| Primer 2 details |
|---|
| Sequence | TTCGACAGGACCTGGGATCT | TCATCTCGGTTTTCCACCCG | Start: 1757 |
| Tm | 59.959 | 60.037 | End: 1987 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | CGCTCTTCCGATCTCGACAA | TGGTCCCAGTAAGGCGAGTA | Start: 44 |
| Tm | 59.901 | 59.96 | End: 298 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | TCTGTTTGCGTTCGACAGGA | TCATCTCGGTTTTCCACCCG | Start: 1747 |
| Tm | 59.897 | 60.037 | End: 1987 |
| Size | 20 | 20 | 241 |
| Primer 5 details |
|---|
| Sequence | AAAACGCGCCCTTTATGCTG | TTTGCCCAGAAATTTGGCCG | Start: 1579 |
| Tm | 60.109 | 59.965 | End: 1809 |
| Size | 20 | 20 | 231 |
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