ssr_primer
Primer Details for TRINITY_DN64714_c2_g1_i1 |
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| (T)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATTCACTCGGCCGGAACTTT | TGCTAAGCAAGAACGCCGTA | Start: 348 |
| Tm | 59.964 | 60.038 | End: 523 |
| Size | 20 | 20 | 176 |
| Primer 2 details |
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| Sequence | TCGCTAGTCGGAAGGCAAAG | TGCTAAGCAAGAACGCCGTA | Start: 317 |
| Tm | 60.109 | 60.038 | End: 523 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
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| Sequence | ATTCACTCGGCCGGAACTTT | CTGCCGTGCTAAGCAAGAAC | Start: 348 |
| Tm | 59.964 | 59.833 | End: 529 |
| Size | 20 | 20 | 182 |
| Primer 4 details |
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| Sequence | GCTCGCAATATTCACTCGGC | TGCTAAGCAAGAACGCCGTA | Start: 339 |
| Tm | 59.767 | 60.038 | End: 523 |
| Size | 20 | 20 | 185 |
| Primer 5 details |
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| Sequence | TCGCTAGTCGGAAGGCAAAG | CTGCCGTGCTAAGCAAGAAC | Start: 317 |
| Tm | 60.109 | 59.833 | End: 529 |
| Size | 20 | 20 | 213 |
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