ssr_primer
Primer Details for TRINITY_DN64698_c1_g1_i1 |
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| (A)23: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGTTGTTGTCGACGAGTA | AAAGGCATTGTTGGTCAGCC | Start: 4 |
| Tm | 60.04 | 59.319 | End: 203 |
| Size | 20 | 20 | 200 |
| Primer 2 details |
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| Sequence | GAAACCGGTTGTTGTCGACG | AAAGGCATTGTTGGTCAGCC | Start: 0 |
| Tm | 60.043 | 59.319 | End: 203 |
| Size | 20 | 20 | 204 |
| Primer 3 details |
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| Sequence | CCGGTTGTTGTCGACGAGTA | AGGGGTAAGAAGGGTAGGCG | Start: 4 |
| Tm | 60.04 | 60.69 | End: 127 |
| Size | 20 | 20 | 124 |
| Primer 4 details |
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| Sequence | GAAACCGGTTGTTGTCGACG | AGGGGTAAGAAGGGTAGGCG | Start: 0 |
| Tm | 60.043 | 60.69 | End: 127 |
| Size | 20 | 20 | 128 |
| Primer 5 details |
|---|
| Sequence | CCGGTTGTTGTCGACGAGTA | GGTAAGCAGGGTAAGAGGGG | Start: 4 |
| Tm | 60.04 | 59.162 | End: 142 |
| Size | 20 | 20 | 139 |
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