ssr_primer
Primer Details for TRINITY_DN64670_c1_g4_i5 |
|---|
| (TTA)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCCCAGAGTGCCAAAAA | GGTGGTAACCGGCTCATGAA | Start: 2066 |
| Tm | 60.033 | 60.036 | End: 2326 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | TTCATGAGCCGGTTACCACC | GCCCGTTTGATAACCAGGGA | Start: 2307 |
| Tm | 60.036 | 60.035 | End: 2491 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | ACACCCCAGAGTGCCAAAAA | GGATTCTTGCACCTGGTCCA | Start: 2066 |
| Tm | 60.033 | 59.962 | End: 2278 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | GGTATTCACCGACCACACGT | TCCTTGTCGTTCAGAGGTGC | Start: 1118 |
| Tm | 60.038 | 59.966 | End: 1359 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | TGGACCAGGTGCAAGAATCC | GCCCGTTTGATAACCAGGGA | Start: 2259 |
| Tm | 59.962 | 60.035 | End: 2491 |
| Size | 20 | 20 | 233 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCCCAGAGTGCCAAAAA | GGTGGTAACCGGCTCATGAA | Start: 2066 |
| Tm | 60.033 | 60.036 | End: 2326 |
| Size | 20 | 20 | 261 |
| Primer 2 details |
|---|
| Sequence | TTCATGAGCCGGTTACCACC | GCCCGTTTGATAACCAGGGA | Start: 2307 |
| Tm | 60.036 | 60.035 | End: 2491 |
| Size | 20 | 20 | 185 |
| Primer 3 details |
|---|
| Sequence | ACACCCCAGAGTGCCAAAAA | GGATTCTTGCACCTGGTCCA | Start: 2066 |
| Tm | 60.033 | 59.962 | End: 2278 |
| Size | 20 | 20 | 213 |
| Primer 4 details |
|---|
| Sequence | GGTATTCACCGACCACACGT | TCCTTGTCGTTCAGAGGTGC | Start: 1118 |
| Tm | 60.038 | 59.966 | End: 1359 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
|---|
| Sequence | TGGACCAGGTGCAAGAATCC | GCCCGTTTGATAACCAGGGA | Start: 2259 |
| Tm | 59.962 | 60.035 | End: 2491 |
| Size | 20 | 20 | 233 |
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