ssr_primer
Primer Details for TRINITY_DN64653_c2_g2_i6 |
|---|
| (A)49: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCGATTGTTTCCCGGCCT | GACTAGCATCCCAGTCTCGC | Start: 556 |
| Tm | 60.034 | 59.969 | End: 658 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | AATCGATTGTTTCCCGGCCT | GCGAGACTAGCATCCCAGTC | Start: 556 |
| Tm | 60.034 | 59.969 | End: 662 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | GACTGGGATGCTAGTCTCGC | TTCTGTGCACGATTCCTCCC | Start: 643 |
| Tm | 59.969 | 60.036 | End: 777 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | GCGAGACTGGGATGCTAGTC | TTCTGTGCACGATTCCTCCC | Start: 639 |
| Tm | 59.969 | 60.036 | End: 777 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | CCCTAGGACGGCAGCATAAG | AACTTGACGGAGCTTCCAGG | Start: 79 |
| Tm | 59.966 | 59.965 | End: 266 |
| Size | 20 | 20 | 188 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCGATTGTTTCCCGGCCT | GACTAGCATCCCAGTCTCGC | Start: 556 |
| Tm | 60.034 | 59.969 | End: 658 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | AATCGATTGTTTCCCGGCCT | GCGAGACTAGCATCCCAGTC | Start: 556 |
| Tm | 60.034 | 59.969 | End: 662 |
| Size | 20 | 20 | 107 |
| Primer 3 details |
|---|
| Sequence | GACTGGGATGCTAGTCTCGC | TTCTGTGCACGATTCCTCCC | Start: 643 |
| Tm | 59.969 | 60.036 | End: 777 |
| Size | 20 | 20 | 135 |
| Primer 4 details |
|---|
| Sequence | GCGAGACTGGGATGCTAGTC | TTCTGTGCACGATTCCTCCC | Start: 639 |
| Tm | 59.969 | 60.036 | End: 777 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | CCCTAGGACGGCAGCATAAG | AACTTGACGGAGCTTCCAGG | Start: 79 |
| Tm | 59.966 | 59.965 | End: 266 |
| Size | 20 | 20 | 188 |
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