ssr_primer
Primer Details for TRINITY_DN64597_c0_g1_i4 |
|---|
| (T)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCCCTTCCAACCTGTTT | GACATCATGTGCCTCCGACA | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1342 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | TGACATCATGTGCCTCCGAC | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1343 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | GTGACATCATGTGCCTCCGA | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1344 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | TCGTGACATCATGTGCCTCC | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1346 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | AGTTTTGACTGTGCGTCCCA | GACATCATGTGCCTCCGACA | Start: 1183 |
| Tm | 60.107 | 60.108 | End: 1342 |
| Size | 20 | 20 | 160 |
(A)11tctttcttttttttgggggc(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACCCCTTCCAACCTGTTT | GACATCATGTGCCTCCGACA | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1342 |
| Size | 20 | 20 | 233 |
| Primer 2 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | TGACATCATGTGCCTCCGAC | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1343 |
| Size | 20 | 20 | 234 |
| Primer 3 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | GTGACATCATGTGCCTCCGA | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1344 |
| Size | 20 | 20 | 235 |
| Primer 4 details |
|---|
| Sequence | CCACCCCTTCCAACCTGTTT | TCGTGACATCATGTGCCTCC | Start: 1110 |
| Tm | 60.106 | 60.108 | End: 1346 |
| Size | 20 | 20 | 237 |
| Primer 5 details |
|---|
| Sequence | AGTTTTGACTGTGCGTCCCA | GACATCATGTGCCTCCGACA | Start: 1183 |
| Tm | 60.107 | 60.108 | End: 1342 |
| Size | 20 | 20 | 160 |
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