ssr_primer
Primer Details for TRINITY_DN64593_c3_g7_i1 |
|---|
| (C)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTCCAAACGTTCACGGA | ATGCTCCACATCGCTGTTGA | Start: 285 |
| Tm | 59.824 | 60.036 | End: 386 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | ACACTCCAAACGTTCACGGA | TGAATGCTCCACATCGCTGT | Start: 285 |
| Tm | 59.824 | 60.036 | End: 389 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGGAGCGTGATCTATGGGTC | TCCGTGAACGTTTGGAGTGT | Start: 83 |
| Tm | 60.039 | 59.824 | End: 304 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CGGAGCGTGATCTATGGGTC | TATCCACCGAAATGCAGGGG | Start: 83 |
| Tm | 60.039 | 59.817 | End: 343 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | GAATTAAAACGGCCCGGAGC | TCCGTGAACGTTTGGAGTGT | Start: 69 |
| Tm | 59.9 | 59.824 | End: 304 |
| Size | 20 | 20 | 236 |
(ATT)4(ATTTA)(TTTA)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTCCAAACGTTCACGGA | ATGCTCCACATCGCTGTTGA | Start: 285 |
| Tm | 59.824 | 60.036 | End: 386 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | ACACTCCAAACGTTCACGGA | TGAATGCTCCACATCGCTGT | Start: 285 |
| Tm | 59.824 | 60.036 | End: 389 |
| Size | 20 | 20 | 105 |
| Primer 3 details |
|---|
| Sequence | CGGAGCGTGATCTATGGGTC | TCCGTGAACGTTTGGAGTGT | Start: 83 |
| Tm | 60.039 | 59.824 | End: 304 |
| Size | 20 | 20 | 222 |
| Primer 4 details |
|---|
| Sequence | CGGAGCGTGATCTATGGGTC | TATCCACCGAAATGCAGGGG | Start: 83 |
| Tm | 60.039 | 59.817 | End: 343 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | GAATTAAAACGGCCCGGAGC | TCCGTGAACGTTTGGAGTGT | Start: 69 |
| Tm | 59.9 | 59.824 | End: 304 |
| Size | 20 | 20 | 236 |
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