ssr_primer
Primer Details for TRINITY_DN64578_c5_g1_i3 |
|---|
| (A)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTCCAAAACGTCCTGGTA | AAACGTCCTTCGCTTTTGGC | Start: 205 |
| Tm | 59.966 | 59.97 | End: 358 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | ATTTCGCCTGCCACCAAGTA | CGCGGCTACAAAAACGGAAA | Start: 926 |
| Tm | 59.962 | 60.041 | End: 1066 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | GCCAAAAGCGAAGGACGTTT | GCCCTGCAGGAAAAAGCAAA | Start: 339 |
| Tm | 59.97 | 59.893 | End: 535 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | CTTTTGCTTCTGCCACCACC | TTGTCACGGACTGCTGGTTT | Start: 2068 |
| Tm | 59.967 | 60.107 | End: 2246 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | GCCAAAAGCGAAGGACGTTT | CAACAGTCTCGGCATGTTGC | Start: 339 |
| Tm | 59.97 | 60.11 | End: 559 |
| Size | 20 | 20 | 221 |
(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGTCCAAAACGTCCTGGTA | AAACGTCCTTCGCTTTTGGC | Start: 205 |
| Tm | 59.966 | 59.97 | End: 358 |
| Size | 20 | 20 | 154 |
| Primer 2 details |
|---|
| Sequence | ATTTCGCCTGCCACCAAGTA | CGCGGCTACAAAAACGGAAA | Start: 926 |
| Tm | 59.962 | 60.041 | End: 1066 |
| Size | 20 | 20 | 141 |
| Primer 3 details |
|---|
| Sequence | GCCAAAAGCGAAGGACGTTT | GCCCTGCAGGAAAAAGCAAA | Start: 339 |
| Tm | 59.97 | 59.893 | End: 535 |
| Size | 20 | 20 | 197 |
| Primer 4 details |
|---|
| Sequence | CTTTTGCTTCTGCCACCACC | TTGTCACGGACTGCTGGTTT | Start: 2068 |
| Tm | 59.967 | 60.107 | End: 2246 |
| Size | 20 | 20 | 179 |
| Primer 5 details |
|---|
| Sequence | GCCAAAAGCGAAGGACGTTT | CAACAGTCTCGGCATGTTGC | Start: 339 |
| Tm | 59.97 | 60.11 | End: 559 |
| Size | 20 | 20 | 221 |
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