ssr_primer
Primer Details for TRINITY_DN64489_c2_g1_i2 |
|---|
| (C)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGTCCTGCATGCAAAAA | CCTCCTTCCTCACCTCTCGA | Start: 777 |
| Tm | 59.968 | 60.034 | End: 901 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | CCACGTCCTGCATGCAAAAA | TCGATACCCCTTTATGCCGC | Start: 777 |
| Tm | 59.968 | 59.966 | End: 885 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | TCTGCATGGAGGTGGACAAC | Start: 360 |
| Tm | 59.964 | 59.963 | End: 498 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | GTCTGCATGGAGGTGGACAA | Start: 360 |
| Tm | 59.964 | 59.963 | End: 499 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AGGGTGTTTCCTGCGATCAG | TCTGCATGGAGGTGGACAAC | Start: 314 |
| Tm | 60.036 | 59.963 | End: 498 |
| Size | 20 | 20 | 185 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGTCCTGCATGCAAAAA | CCTCCTTCCTCACCTCTCGA | Start: 777 |
| Tm | 59.968 | 60.034 | End: 901 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | CCACGTCCTGCATGCAAAAA | TCGATACCCCTTTATGCCGC | Start: 777 |
| Tm | 59.968 | 59.966 | End: 885 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | TCTGCATGGAGGTGGACAAC | Start: 360 |
| Tm | 59.964 | 59.963 | End: 498 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | GTCTGCATGGAGGTGGACAA | Start: 360 |
| Tm | 59.964 | 59.963 | End: 499 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AGGGTGTTTCCTGCGATCAG | TCTGCATGGAGGTGGACAAC | Start: 314 |
| Tm | 60.036 | 59.963 | End: 498 |
| Size | 20 | 20 | 185 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCACGTCCTGCATGCAAAAA | CCTCCTTCCTCACCTCTCGA | Start: 777 |
| Tm | 59.968 | 60.034 | End: 901 |
| Size | 20 | 20 | 125 |
| Primer 2 details |
|---|
| Sequence | CCACGTCCTGCATGCAAAAA | TCGATACCCCTTTATGCCGC | Start: 777 |
| Tm | 59.968 | 59.966 | End: 885 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | TCTGCATGGAGGTGGACAAC | Start: 360 |
| Tm | 59.964 | 59.963 | End: 498 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | TCCGGCGGATGTTTTCTTGA | GTCTGCATGGAGGTGGACAA | Start: 360 |
| Tm | 59.964 | 59.963 | End: 499 |
| Size | 20 | 20 | 140 |
| Primer 5 details |
|---|
| Sequence | AGGGTGTTTCCTGCGATCAG | TCTGCATGGAGGTGGACAAC | Start: 314 |
| Tm | 60.036 | 59.963 | End: 498 |
| Size | 20 | 20 | 185 |
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