ssr_primer
Primer Details for TRINITY_DN64432_c7_g2_i2 |
|---|
| (T)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCCCATGTTGCCTACACT | AATAGCGCGCTTACTCGGAA | Start: 409 |
| Tm | 60.035 | 59.898 | End: 522 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | AAATAGCGCGCTTACTCGGA | Start: 409 |
| Tm | 60.035 | 59.898 | End: 523 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | GCGCTTACTCGGAACTCTCA | Start: 409 |
| Tm | 60.035 | 59.829 | End: 516 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | CAAATAGCGCGCTTACTCGG | Start: 409 |
| Tm | 60.035 | 59.77 | End: 524 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | TCTGTGACAAGCCACGATGT | AGTGTAGGCAACATGGGCTC | Start: 307 |
| Tm | 59.607 | 60.035 | End: 428 |
| Size | 20 | 20 | 122 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGCCCATGTTGCCTACACT | AATAGCGCGCTTACTCGGAA | Start: 409 |
| Tm | 60.035 | 59.898 | End: 522 |
| Size | 20 | 20 | 114 |
| Primer 2 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | AAATAGCGCGCTTACTCGGA | Start: 409 |
| Tm | 60.035 | 59.898 | End: 523 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | GCGCTTACTCGGAACTCTCA | Start: 409 |
| Tm | 60.035 | 59.829 | End: 516 |
| Size | 20 | 20 | 108 |
| Primer 4 details |
|---|
| Sequence | GAGCCCATGTTGCCTACACT | CAAATAGCGCGCTTACTCGG | Start: 409 |
| Tm | 60.035 | 59.77 | End: 524 |
| Size | 20 | 20 | 116 |
| Primer 5 details |
|---|
| Sequence | TCTGTGACAAGCCACGATGT | AGTGTAGGCAACATGGGCTC | Start: 307 |
| Tm | 59.607 | 60.035 | End: 428 |
| Size | 20 | 20 | 122 |
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