ssr_primer
Primer Details for TRINITY_DN64386_c5_g7_i1 |
|---|
| (GCC)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCTACATGCGCGCTTTG | CCTCGTCGTTCACCTCACAA | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1472 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | GTACCTACATGCGCGCTTTG | AACCTCGTCGTTCACCTCAC | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1474 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | GCCGCGGATAATTGTGCATT | Start: 435 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GACGGGTAGGAAAAAGCGGA | GCCGCGGATAATTGTGCATT | Start: 436 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | AGGGCTCTCAACTCGACTCT | Start: 435 |
| Tm | 60.037 | 59.961 | End: 568 |
| Size | 20 | 20 | 134 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCTACATGCGCGCTTTG | CCTCGTCGTTCACCTCACAA | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1472 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | GTACCTACATGCGCGCTTTG | AACCTCGTCGTTCACCTCAC | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1474 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | GCCGCGGATAATTGTGCATT | Start: 435 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GACGGGTAGGAAAAAGCGGA | GCCGCGGATAATTGTGCATT | Start: 436 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | AGGGCTCTCAACTCGACTCT | Start: 435 |
| Tm | 60.037 | 59.961 | End: 568 |
| Size | 20 | 20 | 134 |
(AAAT)6: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTACCTACATGCGCGCTTTG | CCTCGTCGTTCACCTCACAA | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1472 |
| Size | 20 | 20 | 211 |
| Primer 2 details |
|---|
| Sequence | GTACCTACATGCGCGCTTTG | AACCTCGTCGTTCACCTCAC | Start: 1262 |
| Tm | 59.974 | 59.968 | End: 1474 |
| Size | 20 | 20 | 213 |
| Primer 3 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | GCCGCGGATAATTGTGCATT | Start: 435 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
|---|
| Sequence | GACGGGTAGGAAAAAGCGGA | GCCGCGGATAATTGTGCATT | Start: 436 |
| Tm | 60.037 | 59.969 | End: 594 |
| Size | 20 | 20 | 159 |
| Primer 5 details |
|---|
| Sequence | AGACGGGTAGGAAAAAGCGG | AGGGCTCTCAACTCGACTCT | Start: 435 |
| Tm | 60.037 | 59.961 | End: 568 |
| Size | 20 | 20 | 134 |
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