ssr_primer
Primer Details for TRINITY_DN64341_c0_g1_i1 |
|---|
| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACCCCCAATCCATCCCTG | TGAGGCCTAAGCACCCCTAT | Start: 641 |
| Tm | 60.106 | 60.03 | End: 866 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | GAACCCCCAATCCATCCCTG | ATTTGAGGCCTAAGCACCCC | Start: 641 |
| Tm | 60.106 | 60.033 | End: 869 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | CGTTGAATGTTGCGCCATGA | TGAGGCCTAAGCACCCCTAT | Start: 623 |
| Tm | 60.11 | 60.03 | End: 866 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | CGTTGAATGTTGCGCCATGA | ATTTGAGGCCTAAGCACCCC | Start: 623 |
| Tm | 60.11 | 60.033 | End: 869 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | TCAGTCAATGGGTCAGTCGC | CAGGGATGGATTGGGGGTTC | Start: 452 |
| Tm | 60.037 | 60.106 | End: 660 |
| Size | 20 | 20 | 209 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACCCCCAATCCATCCCTG | TGAGGCCTAAGCACCCCTAT | Start: 641 |
| Tm | 60.106 | 60.03 | End: 866 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | GAACCCCCAATCCATCCCTG | ATTTGAGGCCTAAGCACCCC | Start: 641 |
| Tm | 60.106 | 60.033 | End: 869 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | CGTTGAATGTTGCGCCATGA | TGAGGCCTAAGCACCCCTAT | Start: 623 |
| Tm | 60.11 | 60.03 | End: 866 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | CGTTGAATGTTGCGCCATGA | ATTTGAGGCCTAAGCACCCC | Start: 623 |
| Tm | 60.11 | 60.033 | End: 869 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | TCAGTCAATGGGTCAGTCGC | CAGGGATGGATTGGGGGTTC | Start: 452 |
| Tm | 60.037 | 60.106 | End: 660 |
| Size | 20 | 20 | 209 |
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