ssr_primer
Primer Details for TRINITY_DN64315_c2_g1_i1 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTGGCTCCTCGTTGACTTG | CGGACGTTCTGAGTTCACCA | Start: 625 |
| Tm | 59.966 | 59.968 | End: 761 |
| Size | 20 | 20 | 137 |
| Primer 2 details |
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| Sequence | TGCAAGATTCCTTCGGGCAT | CAAGTCAACGAGGAGCCAGT | Start: 415 |
| Tm | 60.034 | 59.966 | End: 644 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
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| Sequence | ACTGGCTCCTCGTTGACTTG | CACCCACCCTGGTTTACCTC | Start: 625 |
| Tm | 59.966 | 59.962 | End: 724 |
| Size | 20 | 20 | 100 |
| Primer 4 details |
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| Sequence | ACTGGCTCCTCGTTGACTTG | AGCGGACGTTCTGAGTTCAC | Start: 625 |
| Tm | 59.966 | 60.319 | End: 763 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
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| Sequence | TGCAAGATTCCTTCGGGCAT | CGCTATTAGCTTTGCGCCAA | Start: 415 |
| Tm | 60.034 | 59.623 | End: 591 |
| Size | 20 | 20 | 177 |
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