ssr_primer
Primer Details for TRINITY_DN64302_c1_g1_i4 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCCCAAAGAAGACTCCGCAT | ACGATCGGCGCGTTAATTTG | Start: 350 |
| Tm | 60.035 | 59.974 | End: 596 |
| Size | 20 | 20 | 247 |
| Primer 2 details |
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| Sequence | TTCCCCAAAGAAGACTCCGC | ACGATCGGCGCGTTAATTTG | Start: 348 |
| Tm | 59.964 | 59.974 | End: 596 |
| Size | 20 | 20 | 249 |
| Primer 3 details |
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| Sequence | CTTAAGTGGGGAGCAGTGGG | CAGCCGCGGAAAAGACAAAA | Start: 2190 |
| Tm | 60.035 | 59.97 | End: 2439 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
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| Sequence | CTTAAGTGGGGAGCAGTGGG | GCCGCGGAAAAGACAAAAGT | Start: 2190 |
| Tm | 60.035 | 59.97 | End: 2437 |
| Size | 20 | 20 | 248 |
| Primer 5 details |
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| Sequence | TGCTTAAGTGGGGAGCAGTG | CAGCCGCGGAAAAGACAAAA | Start: 2188 |
| Tm | 59.963 | 59.97 | End: 2439 |
| Size | 20 | 20 | 252 |
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