ssr_primer
Primer Details for TRINITY_DN64273_c1_g2_i11 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATCGGTGACACAGGCTTT | GTGTGCAGCAAATGAGGCAA | Start: 753 |
| Tm | 59.891 | 59.968 | End: 993 |
| Size | 20 | 20 | 241 |
| Primer 2 details |
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| Sequence | TTTGGACACCGGAAAGCTCA | TAACCGGTGGAGGGACTGAT | Start: 99 |
| Tm | 59.818 | 59.958 | End: 375 |
| Size | 20 | 20 | 277 |
| Primer 3 details |
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| Sequence | GTTCCGGCGTCTCTCAAGAA | GGTAGCCAGAAAAACACCGC | Start: 1570 |
| Tm | 60.039 | 59.76 | End: 1706 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
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| Sequence | TCACGATTTGCAGTGGAGCT | GGTGTCCGTGGAGTTAGCAT | Start: 1052 |
| Tm | 59.965 | 59.751 | End: 1276 |
| Size | 20 | 20 | 225 |
| Primer 5 details |
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| Sequence | TTTGGTCGGTATCGGGGAAC | TTCTTGAGAGACGCCGGAAC | Start: 1453 |
| Tm | 59.75 | 60.039 | End: 1589 |
| Size | 20 | 20 | 137 |
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