ssr_primer
Primer Details for TRINITY_DN64264_c8_g5_i1 |
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| (A)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTGTGAGGAACGCTTGCG | TTGCAGCAATGAAACGGTGG | Start: 1051 |
| Tm | 59.971 | 59.968 | End: 1260 |
| Size | 20 | 20 | 210 |
| Primer 2 details |
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| Sequence | TTTTGTGAGGAACGCTTGCG | GCAATGAAACGGTGGTGCTT | Start: 1051 |
| Tm | 59.971 | 59.968 | End: 1255 |
| Size | 20 | 20 | 205 |
| Primer 3 details |
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| Sequence | TCTGAAGCCGCTGAAGACTG | CGCAAGCGTTCCTCACAAAA | Start: 899 |
| Tm | 60.038 | 59.971 | End: 1070 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
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| Sequence | ATAGTTGGGCCAAAGGGAGC | ATGGCTTCCCGTGAACTGAG | Start: 2386 |
| Tm | 60.033 | 60.036 | End: 2636 |
| Size | 20 | 20 | 251 |
| Primer 5 details |
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| Sequence | TGATGGAAGAGAGGCTCCCA | ACCGCTACACATGATTGCCA | Start: 108 |
| Tm | 59.957 | 60.036 | End: 283 |
| Size | 20 | 20 | 176 |
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