ssr_primer
Primer Details for TRINITY_DN64145_c9_g1_i1 |
|---|
| (G)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCGAGGAATCGAGGTT | GCCACGCCATTGGTGATTAC | Start: 21 |
| Tm | 60.033 | 59.899 | End: 274 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | GCGGACTCTACAACGACCAA | GCCACGCCATTGGTGATTAC | Start: 49 |
| Tm | 60.039 | 59.899 | End: 274 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | TTGGCGGACTCTACAACGAC | GCCACGCCATTGGTGATTAC | Start: 46 |
| Tm | 60.039 | 59.899 | End: 274 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | CCTCCGAGGAATCGAGGTTG | GCCACGCCATTGGTGATTAC | Start: 22 |
| Tm | 59.897 | 59.899 | End: 274 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | TCCTCCGAGGAATCGAGGTT | ACCCCTATCACAGTCGCTCA | Start: 21 |
| Tm | 60.033 | 60.324 | End: 251 |
| Size | 20 | 20 | 231 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCCGAGGAATCGAGGTT | GCCACGCCATTGGTGATTAC | Start: 21 |
| Tm | 60.033 | 59.899 | End: 274 |
| Size | 20 | 20 | 254 |
| Primer 2 details |
|---|
| Sequence | GCGGACTCTACAACGACCAA | GCCACGCCATTGGTGATTAC | Start: 49 |
| Tm | 60.039 | 59.899 | End: 274 |
| Size | 20 | 20 | 226 |
| Primer 3 details |
|---|
| Sequence | TTGGCGGACTCTACAACGAC | GCCACGCCATTGGTGATTAC | Start: 46 |
| Tm | 60.039 | 59.899 | End: 274 |
| Size | 20 | 20 | 229 |
| Primer 4 details |
|---|
| Sequence | CCTCCGAGGAATCGAGGTTG | GCCACGCCATTGGTGATTAC | Start: 22 |
| Tm | 59.897 | 59.899 | End: 274 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
|---|
| Sequence | TCCTCCGAGGAATCGAGGTT | ACCCCTATCACAGTCGCTCA | Start: 21 |
| Tm | 60.033 | 60.324 | End: 251 |
| Size | 20 | 20 | 231 |
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