ssr_primer
Primer Details for TRINITY_DN64117_c3_g1_i1 |
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| (A)10cgataaatctgtgtccaaatgcagcgttgctggagcctagcgata(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTCCAAATGCAGCGTTG | AAGGCAATTCAGACTCCCGG | Start: 351 |
| Tm | 59.969 | 60.035 | End: 486 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
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| Sequence | ATTACAGCGAGCTCGTCAGG | CAACGCTGCATTTGGACACA | Start: 115 |
| Tm | 59.899 | 59.969 | End: 370 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
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| Sequence | ACTGCCCGAGTTGTTTTGGA | CAACGCTGCATTTGGACACA | Start: 218 |
| Tm | 60.107 | 59.969 | End: 370 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
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| Sequence | ACTGCCCGAGTTGTTTTGGA | AAGGCAATTCAGACTCCCGG | Start: 218 |
| Tm | 60.107 | 60.035 | End: 486 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
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| Sequence | CCGGGAGTCTGAATTGCCTT | GCTACTCGACCCACAGTGTC | Start: 467 |
| Tm | 60.035 | 60.109 | End: 635 |
| Size | 20 | 20 | 169 |
(C)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTCCAAATGCAGCGTTG | AAGGCAATTCAGACTCCCGG | Start: 351 |
| Tm | 59.969 | 60.035 | End: 486 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ATTACAGCGAGCTCGTCAGG | CAACGCTGCATTTGGACACA | Start: 115 |
| Tm | 59.899 | 59.969 | End: 370 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | ACTGCCCGAGTTGTTTTGGA | CAACGCTGCATTTGGACACA | Start: 218 |
| Tm | 60.107 | 59.969 | End: 370 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | ACTGCCCGAGTTGTTTTGGA | AAGGCAATTCAGACTCCCGG | Start: 218 |
| Tm | 60.107 | 60.035 | End: 486 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | CCGGGAGTCTGAATTGCCTT | GCTACTCGACCCACAGTGTC | Start: 467 |
| Tm | 60.035 | 60.109 | End: 635 |
| Size | 20 | 20 | 169 |
(A)22: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGTCCAAATGCAGCGTTG | AAGGCAATTCAGACTCCCGG | Start: 351 |
| Tm | 59.969 | 60.035 | End: 486 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | ATTACAGCGAGCTCGTCAGG | CAACGCTGCATTTGGACACA | Start: 115 |
| Tm | 59.899 | 59.969 | End: 370 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | ACTGCCCGAGTTGTTTTGGA | CAACGCTGCATTTGGACACA | Start: 218 |
| Tm | 60.107 | 59.969 | End: 370 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | ACTGCCCGAGTTGTTTTGGA | AAGGCAATTCAGACTCCCGG | Start: 218 |
| Tm | 60.107 | 60.035 | End: 486 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | CCGGGAGTCTGAATTGCCTT | GCTACTCGACCCACAGTGTC | Start: 467 |
| Tm | 60.035 | 60.109 | End: 635 |
| Size | 20 | 20 | 169 |
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